Antigen

Antigen Class

TFs and others

Antigen

DDX5

Cell type

Cell type Class

Pluripotent stem cell

Cell type

NTERA2-derived neural stem cells

NA

NA

Sample

source_name

NTERA2

cell type

NTERA2-derived neural stem cells

chip antibody

DDX5 (Bethyl Laboratories, A300-523A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sheared chromatin was prepared by ChIP-IT Express kit (Active Motif, #53008) and immunoprecipitated by DDX5 or DDX17 antibody. The library preparation was performed using MicroPlex Library Preparation kit (Diagenode, C05010010).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

191148467

Reads aligned (%)

69.6

Duplicates removed (%)

89.0

Number of peaks

46322 (qval < 1E-05)

hg19

Number of total reads

191148467

Reads aligned (%)

68.8

Duplicates removed (%)

90.3

Number of peaks

46417 (qval < 1E-05)