Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
DDX5

Cell type

Cell type Class
Pluripotent stem cell
Cell type
NTERA2-derived neural stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
NTERA2
cell type
NTERA2-derived neural stem cells
chip antibody
DDX5 (Bethyl Laboratories, A300-523A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sheared chromatin was prepared by ChIP-IT Express kit (Active Motif, #53008) and immunoprecipitated by DDX5 or DDX17 antibody. The library preparation was performed using MicroPlex Library Preparation kit (Diagenode, C05010010).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
191148467
Reads aligned (%)
69.6
Duplicates removed (%)
89.0
Number of peaks
46322 (qval < 1E-05)

hg19

Number of total reads
191148467
Reads aligned (%)
68.8
Duplicates removed (%)
90.3
Number of peaks
46417 (qval < 1E-05)

Base call quality data from DBCLS SRA